You are here:   User Profile
Register   |  Login
Profile Avatar
Jahnsens Vei 151
Sem, NA 3170
976 32 463 *******
Currently, public health officials have a hardcore choice to create when it comes to screening people for HIV: administer a dependable blood test that could detect infections in early stages, but that few people will volunteer for, or give people an easy test using saliva that's less reliable during the very first stages of infection. The brand new test could change that.

Undoubtedly the most typical way to try for HIV infection is to try looking in a blood sample for antibodies, proteins that the immunity system custom-builds to attack the virus and fight back against infection. That test is far more convenient than the usual direct seek out the virus, in part because antibodies are relatively abundant in the bloodstream after the early stages of infection.

Yet there is an important drawback, especially for public health officials and researchers who want—and sometimes need—to obtain plenty of people tested quickly to help retain the spread of the condition: needles.

But oral fluid tests have their own problems. While there are HIV antibodies in saliva, they cannot accumulate at the levels they do in blood, or at exactly the same speed—and therefore there just aren't that numerous present, especially early on.

By enough time oral fluid tests can reliably detect HIV, Bertozzi says, "you've waited an extended time"—and in that point, the infection could spread.

The team's job, then, was to find out how to make it easier to detect the small amount of antibodies contained in the saliva of someone with HIV. To achieve that, they took an indirect approach. As opposed to searching for the antibodies themselves, they looked for what antibodies could do.

The team took advantageous asset of a key feature of antibodies—they have two arms, each which easily latches onto a virus like HIV. They took components of HIV and attached them to 1 or one other fifty per cent of a little bit of DNA. Then they added the modified HIV bits into the saliva sample.

If the sample contained HIV antibodies, their two arms would grab your hands on the tagged HIV, bringing the two halves of the DNA together right into a continuous strand. When the DNA piece is manufactured whole it is simple to detect using standard lab techniques.

That may all be done without requiring a blood sample or much technology to process the samples.

Even though the researchers say it can take more studies to confirm the outcomes, the initial experiments show that it works well: the test correctly diagnosed 22 people who took part in an Alameda County screening effort, each of whom had tested positive for HIV using other methods. Importantly, the test did not falsely detect HIV in the 22 additional HIV-negative participants.

It might also work earlier compared to other saliva tests, while not prior to when existing blood tests. In some eight samples that had produced mixed results with the existing standard saliva test, six resulted in positive with the newest HIV test, and one of those was confirmed employing a blood test. Although those email address details are preliminary, they suggest that the newest test is more sensitive and could pick up HIV infection sooner than others.

Beyond HIV, she and Tsai says, the exact same principles may be useful for allergy testing and screening for typhoid and tuberculosis infection. The team can be investigating the strategy as a way to test the efficacy of measles vaccination efforts, Bertozzi says.